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BioMycoX® Mycoplasma PCR Detection KitBioMycoX® Mycoplasma qPCR Detection KitHiSense™ Mycoplasma PCR Detection KitMycoQSearch™ Mycoplasma qPCR Detection KitMycoQSearch™ Plus Mycoplasma qPCR Detection KitBioMycoX® Mycoplasma Elimination KitBioMycoX® Mycoplasma Prevention SprayCryoBanker™ – Cell Freezing MediumMycoplasma 10 CFU StandardMycoplasma Genomic DNAHEX Calibration Plate
BioMycoX® Mycoplasma PCR Detection Kit
This product detects mycoplasma contamination in investigational cell strains through the PCR method.
1. Product Features
- · Mycoplasma contamination screening in R&D cell lines
- · Contains the main contaminants of the cell culture including M. orale, M. hyorhinis, M. arginini, M. fermentans, M. hominis, Acholeplasma laidlawii, allowing all mycoplasma detection of M. pneumoniae, M. salivarium, M. genitalium, M. penentrans and ureaplasma
- · The primer binds to the highly conserved 16s rRNA coding region
- · DNA of eukaryotes and other bacterial species are not detected
- · PCR performance monitoring is possible with the application of internal amplification control (IAC)
- · Optimized PCR Premix provided for easy-to-use
- · Conventional PCR technique applied
- · Template DNA preparation through boiling of the supernatant of the cell culture
- · Sensitivity: 10–100 fg/reaction
- · The test results are within 2~3 hours
2. Product results
Lane 1, 100bp DNA ladder
lane 2, M. pneumoniae DNA 10 pg
lane 3, M. pneumoniae DNA 1 pg
lane 4, M. pneumoniae DNA 100 fg
lane 5, M. pneumoniae DNA 10 fg
lane 6, M. pneumoniae DNA 1 fg
lane 7, Positive control
lane 8, 9, Negative control
lane 10, M. pneumoniae DNA 10 pg
lane 11, M. pneumoniae DNA 1 pg
lane 12, M. pneumoniae DNA 100 fg
lane 13, M. pneumoniae DNA 10 fg
lane 14, M. pneumoniae DNA 1 fg
lane 15, Positive control
lane 16, 17, Negative control
An approximate of 250–300 bp DNA band can be observed at mycoplasma contamination. Furthermore, when PCR reaction is performed appropriately, an internal DNA band of an approximately 700 bp can be identified.
3. Product type
| Type | Product name | Cat. No. | Size | Price (KRW) |
|---|---|---|---|---|
| Mycoplasma screening (R&D) |
BioMycoX® Mycoplasma PCR Detection Kit | D-25 | 25 Tests | 160,000 |
| Mycoplasma screening (R&D) |
BioMycoX® Mycoplasma PCR Detection Kit | D-50 | 50 Tests | 260,000 |
| Mycoplasma screening (R&D) |
BioMycoX® Mycoplasma PCR Detection Kit | D-100 | 100 Tests | 450,000 |
| Mycoplasma screening (R&D) |
SafeDry™ Mycoplasma PCR Detection Kit* | LD-8 | 8 Tests (8 strip x 1 ea) |
80,000 |
| Mycoplasma screening (R&D) |
SafeDry™ Mycoplasma PCR Detection Kit* | LD-48 | 48 Tests (8 strip x 6 ea) |
350,000 |
* SafeDryTM PCR Detection Kit is freeze-dried form of BioMycoX® Mycoplasma PCR Detection Kit.
4. User manual
BioMycoX® Mycoplasma qPCR Detection Kit
This product detects mycoplasma contamination in investigational cell strains through the PCR method.
1. Product Features
- · Mycoplasma contamination screening in investigational cell strains
- · Contains the main contaminants of the cell culture including M. orale, M. hyorhinis, M. arginini, M. fermentans, M. hominis, Acholeplasma laidlawii, allowing all mycoplasma detection of M. pneumoniae, M. salivarium, M. genitalium, M. penentrans and ureaplasma
- · The primer binds to the highly conserved 16s rRNA coding region
- · DNA of eukaryotes and other bacterial species are not detected
- · PCR performance monitoring is possible with the application of internal amplification control (IAC)
- · Optimized PCR Premix provided for easy use
- · Using the TaqMan probe method, real-time PCR (qPCR) technique is applied
(FAM labeled probe: Mycoplasma, HEX labeled probe: IAC) - · Main strand DNA preparation through boiling of the supernatant of the cell culture
- · Sensitivity: 1–100 fg/reaction
- · Result confirmation within 1–2 hr
2. Product results
3. Product type
| Type | Product name | Cat. No. | Size | Price (KRW) |
|---|---|---|---|---|
| Mycoplasma screening (R&D) |
BioMycoX® Mycoplasma qPCR Detection Kit | QDR-25 | 25 Tests | 280,000 |
| Mycoplasma screening (R&D) |
BioMycoX® Mycoplasma qPCR Detection Kit | QDR-50 | 50 Tests | 500,000 |
| Mycoplasma screening (R&D) |
BioMycoX® Mycoplasma qPCR Detection Kit | QDR-100 | 100 Tests | 950,000 |
4. User manual
HiSense™ Mycoplasma PCR Detection Kit
A validation product for biological mycoplasma as well as for mycoplasma contamination in the production cell strain
1. Product Features
- · A validation product for biological mycoplasma as well as for mycoplasma contamination in the production cell strain
- · Contains the main contaminants of the cell culture including M. orale, M. hyorhinis, M. arginini, M. fermentans, M. hominis, Acholeplasma laidlawii, allowing all mycoplasma detection of M. pneumoniae, M. salivarium, M. genitalium, M. penentrans and ureaplasma
- · The primer binds to the highly conserved 16s rRNA coding region
- · DNA of eukaryotes and other bacterial species are not detected
- · PCR performance monitoring is possible with the application of internal amplification control (IAC)
- · Optimized PCR Premix provided for easy use
- · General PCR technique applied
- · Main strand DNA preparation using cell culture solution or commercial DNA extraction kit
- · Sensitivity: 1 ~ 10 fg/reaction
- · Result confirmation within 1–2 hr
- · Passed the Korea Ministry of Food and Drug Safety (MFDS), European Pharmacopoeia (EP), and Japanese Pharmacopoeia (JP) standards
- · UDG system application: Prevention of carry-over contamination
2. Product results
Lane 1, 100bp DNA ladder
lane 2, M. pneumoniae DNA 10 pg
lane 3, M. pneumoniae DNA 1 pg
lane 4, M. pneumoniae DNA 100 fg
lane 5, M. pneumoniae DNA 10 fg
lane 6, M. pneumoniae DNA 1 fg
lane 7, Positive control
lane 8, 9, Negative control
lane 10, M. pneumoniae DNA 10 pg
lane 11, M. pneumoniae DNA 1 pg
lane 12, M. pneumoniae DNA 100 fg
lane 13, M. pneumoniae DNA 10 fg
lane 14, M. pneumoniae DNA 1 fg
lane 15, Positive control
lane 16, 17, Negative control
An approximate of 250–300 bp DNA band can be observed at mycoplasma contamination. Furthermore, when PCR reaction is performed appropriately, an internal DNA band of an approximately 700 bp can be identified.
3. Product type
| Type | Product name | Cat. No. | Size | Price (KRW) |
|---|---|---|---|---|
| Mycoplasma test (QC) | HiSense™ Mycoplasma PCR Detection Kit | HD-50 | 50 Tests | 520,000 |
| Mycoplasma test (QC) | HiSense™ Mycoplasma PCR Detection Kit | HD-100 | 100 Tests | 980,000 |
4. User menual
MycoQSearch™ Mycoplasma qPCR Detection Kit
A validation product for biological mycoplasma as well as for mycoplasma contamination in the production cell strain
1. Product Feature
- · A validation product for biological mycoplasma as well as for mycoplasma contamination in the production cell strain
- · Contains the main contaminants of the cell culture including M. orale, M. hyorhinis, M. arginini, M. fermentans, M. hominis, Acholeplasma laidlawii, allowing all mycoplasma detection of M. pneumoniae, M. salivarium, M. genitalium, M. penentrans and ureaplasma
- · The primer binds to the highly conserved 16s rRNA coding region
- · DNA of eukaryotes and other bacterial species are not detected
- · PCR performance monitoring is possible with the application of internal amplification control (IAC)
- · Optimized PCR Premix provided for easy use
- · Using the TaqMan probe method, real-time PCR (qPCR) technique is applied
(FAM labeled probe: Mycoplasma, HEX labeld probe: IAC) - · Main strand DNA preparation using cell culture solution or commercial DNA extraction kit
- ·Sensitivity: 1 ~ 10 fg/reaction
- · Result confirmation within 1–2 hr
- · Passed the Korea Ministry of Food and Drug Safety (MFDS), European Pharmacopoeia (EP), and Japanese Pharmacopoeia (JP) standards
- · UDG system application: Prevention of carry-over contamination
2. Product results
3. Product type
| Type | Product name | Cat. No. | Size | Price(KRW) |
|---|---|---|---|---|
| Mycoplasma test (QC) | MycoQSearch™ Mycoplasma qPCR Detection Kit (Korea MFDS Ver.) |
QD-50 | 50 Tests | Contact Us |
| Mycoplasma test (QC) | MycoQSearch™ Mycoplasma qPCR Detection Kit (Korea MFDS Ver.) |
QD-100 | 100 Tests | Contact Us |
| Mycoplasma test (QC) | MycoQSearch™ Mycoplasma qPCR Detection Kit (EP Ver.) |
QDEP-50 | 50 Tests | Contact Us |
| Mycoplasma test (QC) | MycoQSearch™ Mycoplasma qPCR Detection Kit (EP Ver.) |
QDEP-100 | 100 Tests | Contact Us |
* QD: The product conforms to the Korea MFDS Guideline.
* QDEP: The product conforms to the Sizes of EP2.6.7.
MycoQSearch™ Plus Mycoplasma qPCR Detection Kit
A validation product for biological mycoplasma as well as for mycoplasma contamination in the production cell strain
1. Product Features
- · A validation product for biological mycoplasma as well as for mycoplasma contamination in the production cell strain
- · Contains the main contaminants of the cell culture including M. orale, M. hyorhinis, M. arginini, M. fermentans, M. hominis, Acholeplasma laidlawii, allowing all mycoplasma detection of M. pneumoniae, M. salivarium, M. genitalium, M. penentrans and ureaplasma
- · The primer binds to the highly conserved 16s rRNA coding region
- · DNA of eukaryotes and other bacterial species are not detected
- · PCR performance monitoring is possible with the application of internal amplification control (IAC)
- · Optimized PCR Premix provided for easy use
- · Using the TaqMan probe method, real-time PCR (qPCR) technique is applied
(FAM labeled probe: Mycoplasma, HEX labeld probe: IAC) - · Main strand DNA preparation using cell culture solution or commercial DNA extraction kit
- ·Sensitivity: 1 ~ 10 fg/reaction
- · Result confirmation within 1–2 hr
- · Passed the Korea Ministry of Food and Drug Safety (MFDS), European Pharmacopoeia (EP), and Japanese Pharmacopoeia (JP) standards
- · UDG system application: Prevention of carry-over contamination
- · Dual probe system: Confirmation for cross-contamination
2. Product results
3. Product type
| Type | Product name | Cat. No. | Size | Price(KRW) |
|---|---|---|---|---|
| Mycoplasma test (QC) | MycoQSearch™ Plus Mycoplasma qPCR Detection Kit (Korea MFDS Ver.) |
QDP-50 | 50 Tests | Contact Us |
| Mycoplasma test (QC) | MycoQSearch™ Plus Mycoplasma qPCR Detection Kit (Korea MFDS Ver.) |
QDP-100 | 100 Tests | Contact Us |
| Mycoplasma test (QC) | MycoQSearch™ Plus Mycoplasma qPCR Detection Kit (EP Ver.) |
QDPEP-50 | 50 Tests | Contact Us |
| Mycoplasma test (QC) | MycoQSearch™ Plus Mycoplasma qPCR Detection Kit (EP Ver.) |
QDPEP-100 | 100 Tests | Contact Us |
* QD: The product conforms to the Korea MFDS Guideline.
* QDEP: The product conforms to the Sizes of EP2.6.7.
BioMycoX® Mycoplasma Elimination Kit
This is a nonantibiotic-type product that permanently eliminates mycoplasma from mycoplasma contaminated cell lines.
1. Product Features
- · Rapidly and permanently eliminates mycoplasma
- · A nonantibiotic-type product created through the combination of biological agents
- · Compensates the demerits (cytotoxicity and antibiotic resistance) of existing antibiotic-type products
- · Applicable to all types of cell lines
- · Nontoxic to most cell lines
- · Elimination range: Mycoplasma, Acholeplasma, Ureaplasma, Spiroplasma, Entomoplasma, and more
2. Mycoplasma elimination results
M: 100bp DNA ladder
1: Positive control (mycoplasma control DNA)
2: Negative control (DW)
3, 6: Mycoplasma infected cell / before treatment
4, 7: Mycoplasma infected cell / 1 passage after treatment
5, 8: Mycoplasma infected cell / 10 passages after treatment
3. Product type
| Type | Product name | Cat. No. | Size | Price (KRW) |
|---|---|---|---|---|
| Reagents | BioMycoX® Mycoplasma Elimination Kit (Light contamination) |
E-01 | 1 Application | 150,000 |
| Reagents | BioMycoX® Mycoplasma Elimination Kit (Medium contamination) |
E-02 | 2 Application | 280,000 |
| Reagents | BioMycoX® Mycoplasma Elimination Kit (Heavy contamination) |
E-03 | 3 Application | 400,000 |
4. User manual
BioMycoX® Mycoplasma Prevention Spray
This is a spray-type product that can effectively prevent mycoplasma contamination.
1. Product Features
- · A spray-type product that can be used easily
- · Completely eliminates mycoplasma within a few seconds
- · Prevents mycoplasma contamination when sprayed and wiped
- · Effective for regular sterilization and cleaning of laboratory equipment and appliances
- · Safe as it is manufactured based on alcohol ingredients
- · The activity of elimination of a wide range of microorganisms
- · Elimination range: Mycoplasma, bacteria, fungi, viruses, etc.
2. Product type
| Type | Product name | Cat. No. | Size | Price (KRW) |
|---|---|---|---|---|
| Spray | BioMycoX® Mycoplasma Prevention Spray | P-100 | 100 ml | 25,000 |
| Spray | BioMycoX® Mycoplasma Prevention Spray | P-500 | 500 ml | 50,000 |
| Spray | BioMycoX® Mycoplasma Prevention Spray | P-1000 | 1L | 80,000 |
| Spray | BioMycoX® Mycoplasma Prevention Spray | P-10000 | 1L X 10 ea | 720,000 |
| Spray | BioMycoX® Mycoplasma Prevention Spray | P-10000R | 1L X 10 ea (For refill) |
67,0000 |
3. User manual
CryoBanker™ – Cell Freezing Medium
This is a product with a composition that does not contain serum components. It can safely freeze and store animal cells while preventing mycoplasma contamination.
1. Product Features
- · 100% serum free
- · 100% mycoplasma free
- · Keeps high viability of cells by freezing them
- · Prevents possible contamination from animal serum-derived microorganisms
- · Maintains uniform performance and stability between batches
- · No additional work required for freezing
- · Makes it possible to freeze cells by putting them immediately into a −80℃ cryogenic freezer
- · No automatic programming device for freezing
2. Product type
| Type | Product name | Cat. No. | Size | Price (KRW) |
|---|---|---|---|---|
| Medium | CryoBanker™ – Cell Freezing Medium | CB-10 | 10 ml | 30,000 |
| Medium | CryoBanker™ – Cell Freezing Medium | CB-50 | 50 ml | 100,000 |
| Medium | CryoBanker™ – Cell Freezing Medium | CB-100 | 100 ml | 180,000 |
3. User manual
Mycoplasma 10 CFU Standard
This is a 10 CFU standard product that can be used for comparative equivalence test in direct mycoplasma culture.
1. Product Features
- · Mycoplasma 10 CFU standard product correctly quantified through a standard test method
- · Safe to use because it is made of mycoplasma dead cells
- · Excludes possible mycoplasma contamination of cell lines used in the laborator
- · All mycoplasma strains in use are purchased from American Type Cell Culture (ATCC)
- · Standard products are manufactured by culturing them in low passage
- · Applicable area: Comparative equivalence test for direct culture
2. Product type
| Type | Product name | Cat. No. | Size | Price (KRW) |
|---|---|---|---|---|
| Standard | Mycoplasma 10 CFU Standard (6 strains) |
M10CFU-6 | 10 CFU | 2,000,000 |
| Standard | Mycoplasma 10 CFU Standard M. arginini |
M10CFU-MA | 10 CFU | 400,000 |
| Standard | Mycoplasma 10 CFU Standard A. laidlawii |
M10CFU-AL | 10 CFU | 400,000 |
| Standard | Mycoplasma 10 CFU Standard M. fermentans |
M10CFU-MF | 10 CFU | 400,000 |
| Standard | Mycoplasma 10 CFU Standard M. hyorhinis |
M10CFU-MH | 10 CFU | 400,000 |
| Standard | Mycoplasma 10 CFU Standard M. orale |
M10CFU-MO | 10 CFU | 400,000 |
| Standard | Mycoplasma 10 CFU Standard M. pneumoniae |
M10CFU-MP | 10 CFU | 400,000 |
Mycoplasma Genomic DNA
This is a product that contains quantified genomic DNA for the sensitivity assessment against mycoplasma.
1. Product Features
- · Mycoplasma DNA standard product correctly quantified through a standard test method
- · Safe to use because it is made of mycoplasma DNA
- · Excludes possible mycoplasma contamination of cell lines used in the laboratory
- · All mycoplasma strains in use are purchased from American Type Cell Culture (ATCC)
- · The DNA is extracted after culturing mycoplasma in low passage
- · Applicable area: Sensitivity test for validation
2. Product type
| Type | Product Name | Cat. No. | Size | Price(KRW) |
|---|---|---|---|---|
| DNA | Mycoplasma Genomic DNA Set (6 strains) |
MGD-6 | 10 ng x 6 | 1,350,000 |
| DNA | M. Arginini Genomic DNA 10 ng | MAGD | 10 ng | 250,000 |
| DNA | A. laidlawii Genomic DNA 10 ng | MLGD | 10 ng | 250,000 |
| DNA | M. fermentans Genomic DNA 10 ng | MFGD | 10 ng | 250,000 |
| DNA | M. hyorhinis Genomic DNA 10 ng | MHGD | 10 ng | 250,000 |
| DNA | M. orale Genomic DNA 10 ng | MOGD | 10 ng | 250,000 |
| DNA | M. pneumoniae Genomic DNA 10 ng | MPGD | 10 ng | 250,000 |
HEX Calibration Plate
This is a calibration product that can be used when HEX dye calibration is needed for appliances in which the MycoQSearch mycoplasma qPCR detection kit is used.
1. An appliance that needs HEX dye calibration
- · Appliance for ABI PCR: StepOne, StepOne plus, 7500, 7500Fast, QuantStudio 3, 5
2. Product type
| Product name | Cat. No. | Size | Price (KRW) |
|---|---|---|---|
| HEX Calibration Plate (96) | QD-HCP96 | 96 wells | 200,000 |
3. User manual