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Product selection guide

DNA Free-Taq polymerase technique by Cellsafe

Most Taq DNA polymerases are contaminated with E. coli DNA, causing the following problems.

  • · Non-specific products from PCR reaction
  • · Primer-dimer formation
  • · Induce false positive result in multiplex PCR
  • · Low sensitivity

  • · Limitation of genetic diagnosis
  • · Incorrect gene cloning
  • · Sequencing error

E. coli DNA contamination test

PCR was performed without template with E. coli 16s rRNA specific primers (1.5 kb product).

MW – 100 bp plus
1) 5 unit DNA free-Taq of CellSafe (30 cycles)
2) 2.5 unit DNA free-Taq of CellSafe (30 cycles)
3) 1.25 unit Taq (A company)
4) 1.25 unit Taq (B company)
5) 1.25 unit Taq (C company)
6) 1.25 unit Taq (D company)

  • · 1, 2: DNA Free-Taq polymerase by CellSafe does not present the band.
  • · 3–6: Taq polymerase by other company presents the band without the template DNA (nonspecific band).

DNA free-Taq polymerase select guide

Product name Routine High Yield Hot Start High CC High Fidelity Long Multiplex
DNA Free-Taq DNA Polymerase O
C-Taq DNA Polymerase O
DNA Free-HotTaq DNA Polymerase O
DNA Free-DuoTaq DNA Polymerase O O
DNA Free-DuoHotTaq DNA Polymerase O O
DNA Free-DLRTaq DNA Polymerase O O
DNA Free-Pfu DNA Polymerase O
DNA Free-Quantum Pfu DNA Polymerase O O
DNA Free-Multiplex Master Mix O O
Product name DNA Free-Taq DNA Polymerase
Routine O
High Yield
Hot Start
High GC
High Fidelity
Long
Multiplex
Product name C-Taq
DNA Polymerase
Routine O
High Yield
Hot Start
High GC
High Fidelity
Long
Multiplex
Product name DNA Free-HotTaq DNA Polymerase
Routine
High Yield
Hot Start O
High GC
High Fidelity
Long
Multiplex
Product name DNA Free-DuoTaq DNA Polymerase
Routine O
High Yield O
Hot Start
High GC
High Fidelity
Long
Multiplex
Product name DNA Free-DuoHotTaq DNA Polymerase
Routine
High Yield O
Hot Start O
High GC
High Fidelity
Long
Multiplex
Product name DNA Free-DLRTaq DNA Polymerase
Routine
High Yield
Hot Start
High GC O
High Fidelity
Long O
Multiplex
Product name DNA Free-Pfu DNA Polymerase
Routine
High Yield
Hot Start
High GC
High Fidelity O
Long
Multiplex
Product name DNA Free-Quantum Pfu DNA Polymerase
Routine
High Yield
Hot Start
High GC
High Fidelity O
Long O
Multiplex
Product name DNA Free-Multiplex Master Mix
Routine
High Yield
Hot Start O
High GC
High Fidelity
Long
Multiplex O

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