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RT-qPCR

HiSense™ QPlex RT-qPCR Master Mix

A one-step product utilizing hydrolysis probe methodology for RNA quantification, it enables reverse transcription and qPCR reactions to be performed consecutively in a single tube.

RTQP-01, RTQP-05

Ordering Information

Cat. No Unit Size Price
RTQP-01 1 ml  Request a Quote→
RTQP-05 5 ml

Product Overview

Features

  • Optimized buffer provide high specificity, robustness and efficiency
  • Use gene specific primers designed to suitable for RNA/cDNA targeting in one-step in a single tube.
  • Easy to analysis of single target in multiple RNA sample
  • Reverse transcription and PCR steps are performed continuously without lid opening to minimize the chance of external contamination
  • MMLV RTase (RNase H minus) can synthesize a very low concentration of RNA
  • Hot-start PCR technique: highly specific amplification

 

 

Results

Amplification plot & Standard Curve

Amplification of GAPDH gene (148bp fragment) of human RNA

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