Product.
CHO (Chinese Hamster Ovary)
ResidualQSearch™ Plus CHO DNA Quantitation Kit
This product is designed for the quantification of residual host cell DNA in pharmaceutical production processes using real-time polymerase chain reaction (real-time PCR or qPCR) with hydrolysis Probe. Chinese Hamster Ovary cell is mammalian cell line that has been widely used in the production of biopharmaceuticals, which are drugs derived from biological sources such as proteins, antibodies, and vaccines.
CHOP-100
Ordering Information
| Cat. No | Unit size | Price |
| CHOP-100 | 100 Tests | Request a quote→ |
Product Overview
Features
- Meets the criteria of the Korean Ministry of Food and Drug Safety (MFDS), World Health Organization (WHO), European Pharmacopoeia (EP), and Janpanese Pharmacopoeia (JP).
- Utilized for quantification of host cell-derived residual DNA in cell culture-derived pharmaceuticals.
- Quantification using real-time PCR (qPCR) technology with hydrolysis probe.
(FAM labeled probe: CHO DNA, HEX labeled probe: IAC) - Digital PCR verified CHO gDNA Standard included in the product.
- Limit of Detection(LOD) : 0.3 fg/reaction
- Limit of Quantitation(LOQ) : 3 fg/reaction
- UDG system : Prevention of carry-over contamination
Results
Amplification Result and Standard Curve
STD1: 3 ng/reaction
STD2: 0.3 ng/reaction
STD3: 30 pg/reaction
STD4: 3 pg/rection
STD5: 0.3 pg/reaction
STD6: 30 fg/reaction
STD7: 3 fg/reaction
Using the ResidualQSearch™ Plus CHO DNA Quantitation Kit, conduct a total of 7-point testing through serial dilution, starting from 3 ng/reaction down to 3 fg/reaction. It was verified to have high sensitivity, quantifying up to 3 fg/reaction.
Support
Certificates of analysis
