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Detection

BioMycoX® Mycoplasma PCR Detection Kit

This kit is designed to detect mycoplasma contamination in research cell lines through conventional PCR.

D-25, D-50, D-100

Ordering Information

Cat. No Unit size Price
D-25 25 Tests Request a quote→
D-50 50 Tests
D-100 100 Tests

Product Overview

Features

  • Screening of mycoplasma contamination in R&D cell cultures
  • Detection of 209 types of mycoplasma with high sensitivity
  • Template DNA: Boiling of culture supernatant or genomic DNA extraction from cultured cells
  • High Sensitivity : 10~100 copies/reaction
  • Specificity : Do not detect other bacterial species with close phylogenetic relation to mycoplsma
  • UDG system : Prevention of carry-over contamination

 

 

Results

Gel Electrophoresis Result
Tested with Quantitative M. arginini Genomic DNA (Cat. No. QAGD, CellSafe)

Lane 1: 100bp ladder
Lane 2: 104 copies
Lane 3: 103 copies
Lane 4: 102 copies
Lane 5: 101 copies
Lane 6: 1 copy
Lane 7: Positive Control
Lane 8: Negative Control

 

  • When mycoplasma contamination exists, a band with around 250-300 bp appears.
  • An internal DNA band with around 700 bp means the right performance of PCR reaction.

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