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Detection

BioMycoX® Mycoplasma PCR Detection Premix

This kit is designed to detect mycoplasma contamination in research cell lines through conventional PCR. It is aliquoted & lyophilized in 8-strip, allowing users to save times and effort conducting experiments.

LD-48

Ordering Information

Cat. No Unit size Price
LD-48 48 Tests Request a quote→

Product Overview

Features

  • Screening of mycoplasma contamination in R&D cell cultures
  • Detection of 209 types of mycoplasma with high sensitivity
  • Template DNA: Boiling of culture supernatant or genomic DNA extraction from cultured cells
  • User friendly : Convenient experimentation by simply adding samples
  • High Sensitivity : 10~100 copies/reaction
  • Specificity : Do not detect other bacterial species with close phylogenetic relation to mycoplsma
  • UDG system : Prevention of carry-over contamination

 

 

Results

Gel Electrophoresis Result
Tested with Quantitative M. arginini Genomic DNA (Cat. No. QAGD, CellSafe)

Lane 1: 104 copies
Lane 2: 103 copies
Lane 3: 102 copies
Lane 4: 101 copies
Lane 5: 1 copy
Lane 6: Positive Control
Lane 7,8: Negative Control
Lane 9: 100bp ladder

 

  • When mycoplasma contamination exists, a band with around 250-300 bp appears.
  • An internal DNA band with around 700 bp means the right performance of PCR reaction.

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