Product.
PCR
HiSense™ Taq PCR Polymerase/ Master Mix/ Premix
This product contains DNA polymerase derived from Thermus aquaticus.
DFT-500, DFT-1000, DFTM-5, LTP-96, LTP-480
Ordering Information
| Type | Cat. No | Unit Size | Price |
| Polymerase | DFT-500 | 500 U | Request a quote→ |
| DFT-1000 | 1000 U | ||
| Master Mix | DFTM-5 | 5 ml | |
| Premix | LTP-96 | 96 Tests | |
| LTP-480 | 480 Tests |
Product Overview
Features
- Source : Thermus aquaticus
- 5’ → 3’ exonuclease activity : Yes
- 3’ → 5’ exonuclease activity : No
- Amplification size : < 3 kb
- A-tailing : Yes
Applications
- Routine PCR
- Allele specific PCR
- 16S and 23S rRNA gene amplification
- Detection of bacteria in samples (e.g. blood)
- DNA labeling reactions & TA-cloning
- Sequencing/ cycle sequencing
Results
The amplification efficiency of HiSense™ Taq Master Mix
The cycling conditions for HiSense™ Taq PCR Master Mix were 95°C for 5 min, 25 cycles of 95°C for 30 sec, 60°C for 30 sec, and 72°C for 1 min.
Lane 1 : 100bp DNA ladder
Lane 2,3 : 10ng human genomic DNA
Lane 4,5 : 2ng human genomic DNA
Lane 6,7 : 0.4ng human genomic DNA
Lane 8,9 : 0.08ng human genomic DNA
Support
Certificates of analysis
Material Safety Data Sheet
