Product.
PCR
HiSense™ HotTaq PCR Polymerase/ Master Mix/ Premix
This product is a mixture of Taq DNA Polymerase and Anti-Taq antibodies. The activity of Taq DNA polymerase is inhibited at room temperature, and the amplification activity is designed to be restored at temperatures above 50°C.
DFH-500, DFHM-5, LHP-96, LHP-480
Ordering Information
| Type | Cat. No | Unit Size | Price |
| Polymerase | DFH-500 | 500 U | Request a quote→ |
| Master Mix | DFHM-5 | 5 ml | |
| Premix | LHP-96 | 96 Tests | |
| LHP-480 | 480 Tests |
Product Overview
Features
- Source : Thermus aquaticus
- 5’ → 3’ exonuclease activity : Yes
- 3’ → 5’ exonuclease activity : No
- Amplification size : < 3 kb
- A-tailing : Yes
- Hot start : Yes
Applications
- SNP/Genotyping
- 16S and 23S rRNA gene amplification
- Detection of bacteria in samples (e.g. blood)
- DNA labeling reactions & TA-cloning
- Minimize primer dimer
Results
Specificity comparison between HiSense™ HotTaq Master Mix and HiSense™ Taq Master Mix
The cycling conditions were 25°C 1hour, 95°C for 5 min, 30 cycles of 95°C for 10 sec, 65°C for 10 sec and 72°C for 30 sec. The Anti-Taq antibody of HiSense™ HotTaq PCR Polymerase prevents the formation of misprimed products and primer-dimers at low temperatures (room temperature).
Lane 1 : 100bp DNA ladder
Lane 2,3 : 1ng human genomic DNA (HotTaq)
Lane 4,5 : 0.1ng human genomic DNA (HotTaq)
Lane 6,7 : 1ng human genomic DNA (Taq)
Lane 8,9 : 0.1ng human genomic DNA (Taq)
Support
Certificates of analysis
Material Safety Data Sheet
