Product.
PCR
HiSense™ DLRTaq PCR Polymerase/ Master Mix/ Premix
This product is optimized for amplifying DNA templates up to a length of 40 kb. The added dUTPase effectively amplifies large-sized DNA by removing dUTP generated at high temperatures.
DLR-500, DFDLM-5, LDLP-96, LDLP-480
Ordering Information
Type | Cat. No | Unit Size | Price |
Polymerase | DLR-500 | 500 U | Request a quote→ |
Master Mix | DFDLM-5 | 5 ml | |
Premix | LDLP-96 | 96 Tests | |
LDLP-480 | 480 Tests |
Product Overview
Features
- 5’ → 3’ exonuclease activity : Yes
- 3’ → 5’ exonuclease activity : Yes (weak)
- Amplification size : < 40 kb
- A-tailing : Yes
Applications
- long-range amplification from genomic DNA
- High amplification efficiency
- Excellent performance on difficult templates
- Amplification of low-copy targets
- High yield and high sensitivity PCR
Results
The amplification efficiency of HiSense™ Taq Master Mix
: The cycling conditions for HiSense™ Taq PCR Master Mix were 95°C for 5 min, 25 cycles of 95°C for 30 sec, 55°C for 30 sec, and 72°C for 1 min.
Lane 1 : 1kb plus DNA ladder
Lane 2,3 : 1kb fragment
Lane 4,5 : 3.8kb fragment
Lane 6,7 : 6.2kb fragment
Lane 8,9 : 7.5kb fragment
Support
Certificates of analysis
Material Safety Data Sheet