Product.
PCR
HiSense™ Multiplex PCR Master Mix/ Premix
This product is designed to simultaneously amplify multiple genes from one or more template DNAs, enabling effective concurrent amplification of amplicons ranging in size from 100 bp to 1 kb.
DFMM-5, LMP-96, LMP-480
Ordering Information
| Type | Cat. No | Unit Size | Price |
| Master Mix | DFMM-5 | 5 ml | Request a quote→ |
| Premix | LMP-96 | 96 Tests | |
| LMP-480 | 480 Tests |
Product Overview
Features
- Source : Thermus aquaticus
- 5’ → 3’ exonuclease activity : Yes
- 3’ → 5’ exonuclease activity : No
- A-tailing : Yes
- Hot start : Yes
- Multiplex PCR : Yes
Applications
- Routine PCR, multiplex PCR and qPCR
- Allele specific PCR
- 16S and 23S rRNA gene amplification
- Detection of bacteria in samples (e.g. blood)
- SNP detection
- Sequencing/ cycle sequencing
Results
5-plex multiplex PCR using HiSense™ Multiplex PCR Master Mix
: The cycling conditions for HiSense™ Multiplex PCR Master Mix were 95°C for 5 min, 25 cycles of 95°C for 30 sec, 55°C for 30 sec, and 72°C for 1 min. The issue of non-specific reaction product formation in Multiplex PCR reactions has been resolved, and technologies such as DNA Free-Taq DNA polymerase and Hot-start have been applied.
Lane 1 : 100bp DNA ladder
Lane 2, 3 : 5-plex multiplex PCR
(100bp, 300bp, 500bp, 800bp, 1000bp)
Lane 4, 5 : 5-plex multiplex PCR
(200bp, 400bp, 700bp, 900bp, 1200bp)
Support
Certificates of analysis
