Product.
RT&RT-PCR
HiSense™ RT-PCR Master Mix/ Premix
HiSense™ RT-PCR Master Mix/Premix is a product that contains all the components necessary for reverse transcription and PCR amplification reactions. Users can perform 'one-step' RT-PCR by adding only template RNA, primer, and RNase free water.
RTPM-1, RTPM-5, LRTP-96, LRTP-480
Ordering Information
| Type | Cat. No | Unit Size | Price |
| Master Mix | RTPM-1 | 1 ml | Request a quote→ |
| RTPM-5 | 5 ml | ||
| Premix | LRTP-96 | 96 Tests | |
| LRTP-480 | 480 Tests |
Product Overview
Features
- The special RT-PCR buffer contains stabilizers and enhancers that optimize the two reactions in a 'one-step'.
- Master mix offers the end-users an efficient, easy to use and reliable alternative to conventional 'two-step' sequencial RT-PCR
- Blue dye is included, making the Master Mix visually confirmable.
- Hot start : Yes
Results
Amplification of GAPDH gene (217bp fragment) of human RNA
The cycling conditions for HiSense™ RT-PCR Master Mix were 42°C for 30 min 95°C for 5 min, 35 cycles of 95°C for 5 sec, 60°C for 30 sec and 72°C for 30 sec. With the inclusion of a special reaction buffer, RTase, RTase inhibitor, dNTPs, and HiSense™ Taq PCR polymerase, high sensitivity and specific RT-PCR are performed.
Lane 1 : 100bp DNA Ladder
Lane 2 : 10ng Human RNA
Lane 3 : 1ng Human RNA
Lane 4 : 100pg Human RNA
Lane 5 : 10pg Human RNA
Lane 6 : 1pg Human RNA
Lane 7 : 100fg Human RNA
Support
Certificates of analysis
Material Safety Data Sheet
