Product.
RT-qPCR
HiSense™ QGreenBlue RT-qPCR Master Mix
HiSense™ QGreenBlue RT-qPCR Master Mix is ready-to-use solution optimized for real-time PCR using SYBR Green type dye. The reverse transcription and PCR steps are performed in one-step assay in a single tube.
RTQBLR-01, RTQBLR-05, RTQBHR-01, RTQBLR-05
Ordering Information
| Type | Cat. No | Unit Size | Price |
| Low ROX | RTQBLR-01 | 1 ml | Request a quote→ |
| RTQBLR-05 | 5 ml | ||
| High ROX | RTQBHR-01 | 1 ml | |
| RTQBHR-05 | 5 ml |
Cat. No. for Instruments (Depending on Rox concentration)
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| Cat. No. | Brand | Model |
| (No ROX) RTQBLR |
BioRad | iCycler, MyiQ, MiQ 2, iQ 5, CFX-96, CFX-384 |
| MJ Research | Opticon, Option2, Chromo4, MiniOpticon | |
| Qiagen | Roto-Gene Q, Roto-Gene3000, Roto-Gene 6000 | |
| Eppendorf | Mastercycler realplex | |
| Illumina | Eco RealTime PCR System | |
| Roche | LightCycler 480, LightCycler 2.0 | |
| (High ROX) RTQBHR |
ABI | 5700, 7000, 7300, 7700, 7900, 7900HT, 7900HT Fast, StepOne, StepOne plus |
| (Low ROX) RTQBLR |
ABI | 7500, 7500 Fast, QuantStudio (1, 3, 5, 6, 7) |
| Stratagene | MX3000, MX3005P, MX4000 |
Product Overview
Features
- Perform reverse transcriptase and qPCR reactions seamlessly in a single tube by simply adding RNA template and primer.
- Verify qPCR results by measuring the real-time fluorescence intensity generated when SYBR Green type dye binds to double-stranded (ds) DNA.
- Hot-start PCR technique: highly specific amplification
- M-MLV Rtase, DNA-free Taq DNA polymerase are included
- The blue dye enables the master mix to be confirmed with the naked eye
Results
Amplification plot & Standard curve
Amplification of GAPDH gene (217bp fragment) of human cDNA
Specificity of HiSense™ QGreenBlue RT-qPCR Master Mix
Support
Certificates of analysis
Material Safety Data Sheet
Product Information
