Product.
PCR
HiSense™ UDG heat labile
HiSense™ UDG Heat-labile contains enzymes derived from a marine bacterium, efficiently preventing carryover contamination in PCR by cleaving DNA at deoxyuridylate residues.
SCU-100
Ordering Information
| Cat. No | Unit Size | Price |
| SCU-100 | 100 ul(1unit/ul) | Request a quote→ |
Product Overview
Features
- Low thermostability and easy inactivation, including enzymes derived from marine bacteria.
- Efficiently hydrolyzes uracil-glycosidic bonds in single- or double-stranded DNA.
- Prevention of carryover contamination in PCR
Results
Efficiency of uracil DNA glycosylase using PCR product (including uracil base)
Lane 1 : 108 copies of a 1KB PCR product with dUTP
Lane 2 : 107 copies of a 1KB PCR product with dUTP
Lane 3 : 106 copies of a 1KB PCR product with dUTP
Lane 4 : 105 copies of a 1KB PCR product with dUTP
Lane 5 : 100bp DNA ladder
Lane 6 : 108 copies of a PCR product with dUTP
Lane 7 : 107 copies of a PCR product with dUTP
Lane 8 : 106 copies of a PCR product with dUTP
Lane 9 : 105 copies of a PCR product with dUTP
Efficiency test of uracil DNA glycosylase was operated using serial diluted PCR products including uracil base. HiSense™ Taq Master Mix was also tested for negative control. Reaction mixture was incubated at 25°C for 5 min followed by 95°C for 5 min, 25 cycles of 30 sec at 95°C, 30 sec at 60°C, 1 min at 72°C. HiSense™ Taq Master Mix (with UDG) contains dUTP besides dATP, dGTP, dCTP and dTTP.
Support
Certificates of analysis
Material Safety Data Sheet
Product Information